Immunoperoxidase technique in histopathology: applications, methods, and controls.
نویسنده
چکیده
منابع مشابه
Application of Specific Monoclonal Antibodies for Characterization of Leishmania spp. Promastigotes Using Indirect Immunofluorescent and Immunoperoxidase Tests
Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA). Methods: Ap...
متن کاملIdentification and Characterization of Leishmania spp. in Impreession Smears of Patients with Cutaneous Leishmaniasis
Background: Monoclonal antibodies have been employed extensively for the identification of Leishmania species, development of diagnostic tests and in the characterization of defined leishmanial antigens. Objectives: Identification and characterization of Leishmania spp. directly from cutaneous lesions of infected individuals. Methods: An immunoperoxidase test (Avidin-Biotin technique) using mo...
متن کاملThe frequency of vascular changes in the histopathology of cutaneous leishmaniasis
Introduction: Cutaneous leishmaniasis (CL) is a parasitic skin infection which can be debilitating. This study was performed to determine the vascular changes in the histopathology of CL. Methods: Thirty-seven patients with CL were included in a prospective study. A 4 mm punch biopsy was obtained from the border of the lesion. After histopathologic processing and H&E staining, it was reviewed ...
متن کاملImmunohistochemical staining for megakaryocyte c-mpl may complement morphologic distinction between polycythemia vera and secondary erythrocytosis.
Recent studies have shown decreased megakaryocyte expression of the thrombopoietin receptor (c-mpl) in patients with polycythemia vera (PV) but not in those with reactive erythrocytosis. We examined the diagnostic utility of this observation in 22 patients with PV, 7 patients with secondary erythrocytosis (SE), and 10 normal controls. Commercial antibodies against c-mpl were used with standard ...
متن کاملImmunohistochemical expression of p53 in animal tumors: a methodological study using four anti-human p53 antibodies.
Mutations in the p53 tumor suppressor gene are the most common genetic alterations in human cancers. These mutations usually lead to strongly enhanced protein stabilization and allow detection by immunohistochemistry. Two monoclonal (DO-7 and PAb-240) and two polyclonal (Ab-7 and CM-1) antibodies were evaluated by standard immunoperoxidase method in domestic animal tumors, chiefly squamous cell...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 32 10 شماره
صفحات -
تاریخ انتشار 1979